P E C S > B V - 1 0
0.1um through 50um finished electrodes depending on abrasive plate
less than 1.0um
5-90 degrees - adjustable
0.075in / dial revolution
0.0004in / dial revolution
19in x 9in x 8in
48cm x 22cm x 20cm
50/60 Hertz power line
Elegant and simple to use, the BV-10
offers precision beveling of micropipette tips between 0.1 and 100µm. The
unique abrasive plate drive system is vibration free for greater control
of the beveling process. Beveling can be accomplished very rapidly and
produces consistent tip diameters using the techniques as described by
Brown and Flaming, Science, August 1974, Vol. 185.
E A T U R E S > B V - 1 0
magnetically coupled beveling surface.
surface optically flat to a half wave (250nm).
clock motor insures stable rotation rate.
steel baseplate adds additional dampening.
micromanipulator controls bevel angle and advancement.
Intracellular recording electrodes can benefit from beveling
because of 1) a reduction in the tip diameter by creation of the sharp
point on the electrode and 2) a lowered electrical resistance of the electrode
due to the larger cross sectional area of the lumen. This greatly facilitates
penetrating and holding very small or difficult cells. Microinjection
needles also benefit from beveling by promoting entry into cells with
minimal damage while at the same time enhancing the flow of material through
The basic beveling system consists of a stationary pedestal, optically
flat to a half wave (250nm), surface mounted on a heavy baseplate. This
serves as a bearing for an abrasive coated glass grinding plate, which
is also flat to half a wave. The flat abrasive plate is coupled to a low
vibration, slow-speed motor by means of magnetic fields to provide a wobble-free
flat grinding surface. The abrasive plates are fabricated with a proprietary
process which insures a consistent abrasive coating.
A 2-stage micromanipulator holds the pipette to be beveled and permits
controlled advancement onto the abrasive surface. The bevel angle and
speed of advancement are adjustable. A halogen lamp with a gooseneck enhances
the beveling operation by providing sharp illumination of the abrasive
plate and pipette.
The basic system is completed with two abrasive plates of your choice,
a wick with holder (for wet beveling), pedestal oil, degreasing fluid,
Two options are available for monitoring the beveling process, an 80X,
stereo microscope and an electrode impedance meter. Depending on your
research application, one or both of these options may be desirable. For
all micropipette applications, the swing mounted microscope enhances your
control of pipette advancement onto the abrasive plate and allows for
viewing of the beveling operation (scope resolution is not sufficient
for viewing the actual bevel except in the case of very large tips). For
microelectrode applications, the impedance meter is used to monitor the
tip resistance during the beveling operation. The meter is an analog design,
offering three resistance ranges (0-10, 0-100, 0-500 MOhm). Measurements
are made at 12 Hz to minimize capacitive contributions to the impedance
measured and provide a near-true DC resistance value. A rapid roll-off
is used to reduce 50/60 Hz interference, allowing operation in a laboratory
environment without screening.