If you do not see your concern listed below, you may receive additional advice by contacting an engineer who is knowledgeable about your instrument, emailing tech@sutter.com or placing a call to 415.883.0128. Please note that we are located in California, USA and our normal business hours are 8:00am to 5:00pm Pacific Time, GMT-8.

Our technical support engineers and the products they handle:
Adair Oesterle - P-1000, P-97, P-30, XenoWorks Microinjection Systems, BV-10 Microelectrode Beveler, (Discontinued Products: P-87, P-80, P-80PC, P-77)

Ali Mahloudji - Micromanipulators, Lambda DG-4/DG-5/DG-4 Plus, XenoWorks, Translators, MP Stages

Chris Ballard - Lambda XL, Lambda LS, Microscope Stages and Translators

Dan Carte - Lambda 10-3, Lambda 10-2, Lambda 10-B & discontinued Lambdas, SmartShutter™, Optical filter changers, OEM filter wheels

Jack Belgum - P-2000

Rick Ayer - Moving Objective Microscope (MOM), Lambda LS

Aaron Best - Moving Objective Microscope (MOM)

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MICROPIPETTE FABRICATION

 Pullers Troubleshooting Pipette Variablity (video) New
Puller Bars and V-groove Bearings (video) New
Checking the Pull Cables (video) New
Checking the Cable Pulleys (video) New
 P-2000 P-2000 Introduction (video) New
Pulling A Patch Pipette From Quartz Glass (video) New
Effects of The Velocity Parameter (video) New
Copying Pull Programs on the P-2000 Puller (video) New
Information on the new style P-2000 "leveled" puller bar
P-2000 Glass Loading Aid
P-2000 Glass Stop
P-2000 New style glass clamp

 P-1000 Copying Pull Programs on the P-1000 Puller (video) New
 P-97/P-87 How do I run a Ramp Test?
Troubleshooting variability.
If you need to call in for assistance, please print and answer the questions on this Variability Questions Check List and have it on hand when contacting Sutter Instrument Technical Support.
Troubleshooting problems with burning out filaments.
Regeneration of Drierite (Blue Granules)
Diagnosing air leak or blockage problems
Adjusting Filament Position Using Eccentrics

 Pipette Shape Pipette Cookbook (6MB file)
Changing Pipette Shape
Pronuclear Injection Needle
Patch Pipettes
25 - 35 micron tip with a Short taper (3-4mm)
The Sutter Ceramic Tile & making a 20-200 micron tip with a 5 to 15mm long taper

 Filament Concerns Replacing, Centering, and other filament concerns

 P-80/PC

Using a P-97 manual with a P-80PC
P-80PC General Concerns


MICROINJECTION

 XenoWorks Microinjection Applications
How would I deliver genetic material to mammalian cells by microinjection?
Using the Analog Injector (BRI) with a Three-way Valve
BRI syringe size and volume delivery table
BRE Digital Injector pressure settings

 Manual Microinjector Filling the Manual Injector with Oil or Water